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KMID : 1094720210260030419
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Biotechnology and Bioprocess Engineering
2021 Volume.26 No. 3 p.419 ~ p.426
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KaAhl, a Novel N-Acylhomoserine Lactonase from Kushneria avicenniae and Attenuated Effect on the Virulence of Erwinia carotovora
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Su Yue
Shao Mengru
Li Tianying
Lin Ling
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Abstract
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N-acylhomoserine lactones (AHLs) are autoinducers found in gram-negative bacteria and are ubiquitous in common plant pathogenic bacteria. N-acylhomoserine lactonase degrades the lactone ring of AHLs to achieve quorum quenching of phytopathogenic virulence genes. In this study, a novel AHL lactonase gene KaAhl was successfully cloned and identified from Kushneria avicenniae strain DSM 23439. KaAhl is encoded a 261-residue polypeptide belonging to the metallo-β-lactamase superfamily. The recombinant enzyme displayed maximum hydrolysis activity of 0.64 U/mg toward N-(3-oxooctanoyl)-L-homoserine lactone substrate at 30°C and pH 8.0. Besides, it had moderate thermal stability but exhibited a high salt tolerance showing approximate 50% relative activity in the presence of 35% concentrations of NaCl. Furthermore, the KaAhl was significantly promoted by Mn2+ and Ni2+, which enhanced the lactonase activity to approximate 100% extent. In addition, it was observed that recombinant Escherichia coli BL21-pET28a-KaAhl producing KaAhl proteins could effectively inhibit the plant pathogenicity of Erwinia carotovora for 72 h, which might have great potential for controlling gram-negative pathogenic bacteria.
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KEYWORD
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N-acylhomoserine lactonase, Kushneria avicenniae, quorum quenching, soft rot disease
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